Translation

Translation


3 Processes of Translation

  1. Initiation
  2. Elongation
  3. Termination

Transcription is the process of RNA being read and converted to a polypeptide chain of proteins. First, there are several major factors.


Factors Function
Ribosome
  • Comprised of 2 subunits: large and small
  • Builds the protein
  • Provides a spot for the tRNA and mRNA to interact
  • Performs enzyme dehydration synthesis
  • Reads in three RNA nucleotides at once
tRNA
  • Shaped in a specific "T" structure
  • Has an anticodon at the bottom tip
  • Amino acid attached to upper half
  • Translates an individual 3-letter codon in the mRNA
Release factor
  • A protein that tells the ribosome to stop translating
P-site and A-Site
  • Where the tRNAs come in

Initiation

  1. The smallest subunit of the ribosome attaches to the 5' cap
  2. The large subunit then attaches to the small subunit which forms an entire ribosome
  3. The ribosome moves along until it finds the AUG codon

Elongation

  1. A tRNA with an anticodon that matches the AUG codon flies into the A-Site
  2. The ribosome takes the amino acid on the end of the tRNA and holds it
  3. The first tRNA goes to the P-Site and the ribosome moves on to the next 3 RNA nucleotides
  4. A tRNA with that anticodon goes into the A-Site
  5. The ribosome takes the amino acid of the new tRNA and attaches it to the one it is holding
  6. The AUG anticodon tRNA goes out of the ribosome to get a new amino acid
  7. Meanwhile, the new tRNA that just came in goes to the P-site and the ribosome reads the next three RNA nucleotide
  8. This eventaully creates a long chain of polypeptide which is the primary structure of a protein

Termination

  1. After reading through the mRNA, the ribosome encounters a release factor
  2. The release factor tells the ribosome to stop translating and pulling tRNAs (IMPORTANT NOTE: The release factor does not have an amino acid associated with it and thus does not get translated)
  3. Both subunits of the ribosome falls off and goes to find another mRNA

And the the protein folds while the mRNA is broken down by enzymes and reused in the nucleus.


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